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Accession_number |
ACC 1
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Cell_line_name |
P-815
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Brief_description |
confirmed as mouse with IEF of LDH, NP, PEP B established from the mastocytoma
tumor of a DBA/2 mouse treated with methylcolanthrene; used as target cells for
cytotoxic T cell assays; as reported cells exhibit no effector activity in an
antibody-dependent cell mediated cytotoxic system
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Description |
mouse mastocytoma established from the mastocytoma tumor of a DBA/2 mouse
treated with methylcolanthrene; used as target cells for cytotoxic T cell assays;
as reported cells exhibit no effector activity in an antibody-dependent cell
mediated cytotoxic system confirmed as mouse with IEF of LDH, NP, PEP B
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Depositor |
Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
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Bibliographic_reference |
Ralph et al., Biochem. Biophys. Res. Commun. 61: 1268-1275 (1974); Ralph et al.,
Nature 249: 49-51 (1974); Ralph et al., J. Exp. Med. 143: 1528-1553 (1976);
Ralph et al., J. Immunol. 119: 950-954 (1977)
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Morphology |
single, round cells in suspension; some cells may be adherent
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Culture_conditions |
90% RPMI 1640 + 10% FBS maintain at 0.5-5 x 105 cells/ml, cells grow very
fast; split 1:5 to 1:10 every 3-4 days; seed out at ca. 1-2 x 106 cells/10 ml
at 37C with 5% CO2 maximum cell density at > 1 x 106 cells/ml
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Viruses |
ELISA: reverse transcriptase negative
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Properties |
-
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Release_conditions |
General restrictions
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule
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Doubling_time |
doubling time of ca. 18-22 hours
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Mycoplasma |
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
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Cytogenetics |
murine near-diploid karyotype; 41(36-43)<2n>; 1-2 centric fusion markers present
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