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ICLC Animal Cell Lines Database Address IRCCS Ospedale Policlinico San Martino L.go R. Benzi, 10 16132 Genova ITALIA Phone +39 010 555 7491 - 7492 Fax No fax available Email [email protected] WWW http://www.iclc.it/ Conditions The cell lines will be made available subject to the following conditions: 1. The cultures are to be used either by: � Scientist affiliated to a non-profit research institute in pursuance of an academic research programme or � if by commercial or similar interests, they will not be offered for sale or be utilized in commercial processes involved in the preparation for sale of any biological material, or in any other type of commercial activity without having secured written agreement from the ICLC on behalf of the original depositor. The ICLC will refer back to depositors all such requests. 2. The cell lines will not be distributed to third parties. 3. If the cell lines are referred to in any publication, then correct reference will be made to the work of the original depositor and no alteration will be made to its ICLC cell line name or acronym. 4. The ICLC and its depositors do not accept any liability in connection with the handling or use of the cell line. Shipping information The cells will be shipped on mondays, in the week following the receival of the order. In case a technical problem would arise, the customer will be promptly informed. Contact Person Barbara Parodi Additional Database Information ICLC Example of an entry from the ICLC catalogue. Accession_number ICLC AL96001 Cell_line_name SIRC Brief_description Species: rabbit; Tissue: cornea Description Species: rabbit; Tissue: cornea Depositor Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy. Bibliographic_reference Proc Soc Exp Biol Med 1970;135:1543-1545 Morphology fibroblast-like, grown as monolayer Culture_conditions continuous culture; MEM (EBSS) + 10% FBS + 1% non-essential amino acids; Split confluent cultures 1:4-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. Viruses There is no evidence for the presence of infectious viruses Properties virus replication; not susceptible to poliovirus2, Coxsackie A9 and B5; Susceptible to: rubella Release_conditions Cell line available for distribution. For non-commercial investigative use only Hazard Cell line of non primate origin. There is no evidence for the presence of infectious viruses or toxic products. However, it is recommended that cultures are handled under Biosafety Level 2 containment (Italian Decree Law 626/94). Passage_number 12 Species_validation Validated by isoenzymes: confirmed as rabbit with AST, G6PD, PepB Karyology modal number 66 Freezing_medium Culture medium + 50% FBS + 10% DMSO Sterility mycoplasma negative, HOECHST and PCR Further_bibliography Proc Soc Exp Biol Med 1966;122:783 [PMID: 5918951] Proc Soc Exp Biol Med 1967;125:1271 [PMID: 6042441] Virology 1984;132:79-84 [PMID: 6607566] Top of Page © The CABRI Consortium 1999-2020. This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium. Site maintained by Paolo Romano. Last revised in January 2020.